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KMID : 0376119940210020243
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Medical Journal of the Red Cross Hospital
1994 Volume.21 No. 2 p.243 ~ p.251
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Morphological Study of Surgically Induced Open Neural Tube Defect in Early Chick Embryos
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Abstract
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For the experimental stud of neural tube defect (NTD), surgical model has advantages over other models in some aspects. It causes less functional derangement of cells and the defect can be made selectively by surgery. the authors planned to use
the
surgical model for the experimental study of NTD. As the first step for the studies, the chronological changes of morphology during the early postoperative period were investigated using post-incubation 3 day chick embryos. The objects of this
study are
1) the morphological evaluation of surgically induced open NTD model as a method for the first 24 hours after surgery which include 'overgrowth' appearance and the continuity between the surgace ectoderm and the neuroectoderm. The morphologic
changes
were observed with the light microscope and the scanning electron microscope.
Immediately after surgery, a typical open NTDs were observed. Morphologically they were very similar to the appearance of spontaneous open NTDs. The size of the defect increased in both upper and lower ends for the 24 hours. Cellular hyperplasia
(overgrowth appearance) was noted immediately after surgery in both sides of the NTD, and became more prominent during the 24 hours. There was increasing continuity between the surface ectoderm and the neural tissue until 24 hours after surgerv
when the
continuity looked almost complete.
In conclusion, surgically induced NTDs are morphologically very similar to the spontaneous NTDs. Overgrowth appearance and the continuity between the surface ectoderm and the neural tissue at the site of NTD, once thought as evidences supporting
'failure to close' theories, should not be considered so because they were also observed in this 'reopen' model.
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KEYWORD
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